CD3 epsilon anticorps (Cytoplasmic Domain)

N° du produit ABIN4949516

Cet anticorps anti-CD3 epsilon est un anticorps Souris Monoclonal détectant CD3 epsilon dans FACS, WB, IF et IHC (p). Adapté pour Humain, Souris, Rat, Porc, Boeuf (Vache), Cheval, Lapin et Cobaye.

Aperçu rapide pour CD3 epsilon anticorps (Cytoplasmic Domain) (ABIN4949516)

Antigène: CD3 epsilon (CD3E)

Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Cheval, Lapin, Cobaye

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 epsilon est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PC3-188A

Détail du produit anti-CD3 epsilon anticorps

Épitope: AA 156-168, Cytoplasmic Domain

Purification: Protein G affinity chromatography

Immunogène: Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.

Isotype: IgG1

Information d'application

Indications d'application: Optimal dilution of the CD3e antibody should be determined by the researcher.\. WB: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT,Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3e

Sujet: Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28  kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway